pltp  (Boster Bio)

Journal: Critical Care

Article Title: The role of phospholipid transfer protein in sepsis-associated acute kidney injury

doi: 10.1186/s13054-025-05253-6

Figure Lengend Snippet: Characteristics of patients at ICU admission

Article Snippet: The human recombinant PLTP was sourced from Pharmaron Inc.

Techniques: Activity Assay

Journal: Critical Care

Article Title: The role of phospholipid transfer protein in sepsis-associated acute kidney injury

doi: 10.1186/s13054-025-05253-6

Figure Lengend Snippet: Association of PLTP lipotransfer activity with SA-AKI and MAKE30. SA-AKI: adjusted for age, hypertension, CKD, Apache II, PCT, HDL-C, LDL-C, APOA1, TC, lactate, BE, platelet, PT, and ALT. MAKE30: adjusted for age, hypertension, CKD, CAD, Apache II, PCT, HDL-C, LDL-C, APOA1, TC, lactate, BE, and PT.SA-AKI, sepsis-associated acute kidney injury; MAKE30, major adverse renal event within 30 days; PLTP, phospholipid transfer protein

Article Snippet: The human recombinant PLTP was sourced from Pharmaron Inc.

Techniques: Activity Assay

Journal: Critical Care

Article Title: The role of phospholipid transfer protein in sepsis-associated acute kidney injury

doi: 10.1186/s13054-025-05253-6

Figure Lengend Snippet: The predictive value of PLTP lipotransfe activity for SA-AKI and MAKE30 ( A ) and differences in cumulative incidence of MAKE30 by PLTP lipotransfer activity level ( B ). SA-AKI, sepsis-associated acute kidney injury; MAKE30, major adverse renal event within 30 days; PLTP, phospholipid transfer protein

Article Snippet: The human recombinant PLTP was sourced from Pharmaron Inc.

Techniques: Activity Assay

Journal: Critical Care

Article Title: The role of phospholipid transfer protein in sepsis-associated acute kidney injury

doi: 10.1186/s13054-025-05253-6

Figure Lengend Snippet: Low expression of PLTP correlated with decreased kidney function. A Plasma PLTP lipotransfer activity in the indicated groups, n = 3–4. B Expression of PLTP mRNA in kidney tissues from the indicated groups, n = 3–4. C Western blot analysis for kidney PLTP and GAPDH in different groups along with quantification of western blotting, n = 3–4. D Immunohistochemistry of kidney PLTP in the indicated groups, n = 3–4. E Survival rates of mice after CLP, n = 6. Survival rates were analyzed by Kaplan–Meier method and compared by log-rank test. F Plasma TNF-α in different groups, n = 5. G Plasma IL-6 in the indicated groups, n = 5. H Plasma creatinine in the indicated groups, n = 5. I Plasma BUN in the indicated groups, n = 5. J Expression of NAGL mRNA in kidney tissues of different groups, n = 5. K The expression level of Kim-1 mRNA in kidney tissues of different groups, n = 5. L Hematoxylin and eosin staining along with tubular injury scoring of kidneys in different groups, green arrows indicate tubular dilatation, blue arrows represent epithelial cell detachment from tubular lining, and black arrows represent mononuclear inflammatory cell infiltration, n = 5. Data are shown as the mean ± SD, and statistical significance was assessed by t-test analysis

Article Snippet: The human recombinant PLTP was sourced from Pharmaron Inc.

Techniques: Expressing, Activity Assay, Western Blot, Immunohistochemistry, Staining

Journal: Critical Care

Article Title: The role of phospholipid transfer protein in sepsis-associated acute kidney injury

doi: 10.1186/s13054-025-05253-6

Figure Lengend Snippet: RhPLTP can reduce mortality and ameliorate kidney injury in WT CLP mice. A Flowchart of rhPLTP injection protocol. B Injection of rhPLTP increased the survival rates after CLP. Mice were administered with PBS (control) or rhPLTP (25 µg in a volume of 200 µl) 1 h, 7 h, and 23 h before CLP, n = 9. Survival rates were analyzed by the Kaplan–Meier method and compared using the log-rank test. C Plasma IL-6 in different groups, n = 6. D Plasma TNF-α in different groups, n = 6. E Plasma creatinine in different groups, n = 6. F Plasma BUN in different groups, n = 6. G Expression of NAGL mRNA in kidney tissues of different groups, n = 6. H Expression of Kim-1 mRNA in kidney tissues of different groups, n = 6. I Hematoxylin and eosin staining along with PLTP immunohistochemistry of the different groups, n = 6. J The representative ultrastructure images of mouse renal epithelial cells observed by transmission electron microscopy; red arrows represent disordered arrangement of mitochondria in the renal cortical tubular epithelial cells, edema, reduction and disruption of mitochondrial cristae, vacuolar degeneration is observable; green arrows indicate that in the renal cortex, mitochondrial edema is still observable in the mitochondria, with reduction, disruption and partial disappearance of mitochondrial cristae, n = 6. Data are shown as the mean ± SD, and statistical significance was assessed by t-test analysis

Article Snippet: The human recombinant PLTP was sourced from Pharmaron Inc.

Techniques: Injection, Control, Expressing, Staining, Immunohistochemistry, Transmission Assay, Electron Microscopy, Disruption

Journal: Nutrition Research and Practice

Article Title: Ellagic acid, a functional food component, ameliorates functionality of reverse cholesterol transport in murine model of atherosclerosis

doi: 10.4162/nrp.2024.18.2.194

Figure Lengend Snippet: CETP, cholesterol ester transfer protein; PLTP, phospholipid transport protein; LCAT, lecithin-cholesterol acyltransferase; PON1, paraoxonase-1; WT, wild type; apoE KO, apolipoproteins E knockout; ELISA, enzyme-linked immunosorbent assay.

Article Snippet: Plasma levels of CETP (MyBioSource, San Diego, CA, USA), PLTP (MyBioSource), and LCAT (Cloud-Clone, Katy, TX, USA) were determined by using commercial sandwich-type ELISA kits.

Techniques: Knock-Out, Enzyme-linked Immunosorbent Assay